Interactions of Soluble Recombinant Integrin αvβ5 with Human Adenoviruses
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منابع مشابه
Interactions of Soluble Recombinant Integrin avb5 with Human Adenoviruses
av integrins have been identified as coreceptors for adenovirus (Ad) internalization; however, direct interactions of these molecules with Ad have not been demonstrated. We report here the expression of soluble integrin avb5, which retains the ability to recognize the Ad penton base as well as vitronectin, an Arg Gly Asp (RGD)-containing extracellular matrix protein. Soluble integrin avb5 react...
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p21-activated kinase 1 (PAK1) can affect cell migration (Price et al., 1998; del Pozo et al., 2000) and modulate myosin light chain kinase and LIM kinase, which are components of the cellular motility machinery (Edwards, D.C., L.C. Sanders, G.M. Bokoch, and G.N. Gill. 1999. Nature Cell Biol. 1:253-259; Sanders, L.C., F. Matsumura, G.M. Bokoch, and P. de Lanerolle. 1999. SCIENCE: 283:2083-2085)....
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Endocytosis and degradation of vitronectin by human skin fibroblasts are regulated by the β5 integrin. To determine whether the β5 integrin is directly mediating the internalization of vitronectin, both vitronectin and the β5 integrin were localized by indirect immunofluorescence during the endocytic process. This analysis showed that both vitronectin and β5 were found in intracellular vesicles...
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Recombinant human Glutaminyl Cyclase expressed in E. coli is produced as inclusion bodies. Lack of glycosylation is the main origin of its accumulation in insoluble aggregates. Mutation of single isolated hydrophobic amino acids into negative amino acids was not able to circumvent inclusion bodies formation. On the contrary, substitution with carboxyl-terminal residues of two or three aromatic ...
متن کامل[Effect of recombinant adenoviruses with CD/TK fusion suicide gene on human hepatocellular carcinoma cells].
OBJECTIVE To investigate gene-therapy for human hepatocellular carcinoma with adenovirus vectors by double suicide gene CD/TK. METHODS Double suicide gene CD/TK was liberated from eukaryotic vectors pCEA-CD/TK and subcloned into shuttle vectors, and the transfer plasmid pAdtrack-CMV-CD/TK was formed after linearizing with Pac 1. It was recombinated with pAdeasy-1 in bacteria BJ5183. The ident...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1998
ISSN: 1098-5514,0022-538X
DOI: 10.1128/jvi.72.11.8669-8675.1998